目的 揭示不同病程2型糖尿病(T2DM)肾病大鼠肾动脉上大电导钙激活钾通道基因表达是否有改变。方法 采用单肾切除+小剂量链脲佐菌素(streptozotocin,STZ)+高脂饮食复制T2DM大鼠肾病模型,采用实时定量聚合酶链反应(PCR)法测定肾动脉BKCa通道的ɑ、β1亚单位的mRNA表达水平,同时全自动血生化仪检测血尿素氮、血肌酐;收集24 h尿蛋白定量;计算肾脏指数;肾组织切片光、电镜观察病理改变。结果 8、12周T2DM肾病大鼠血糖,血胰岛素,血肌酐,尿素氮,24 h尿蛋白,肾脏肥大指数均明显高于对照组(P<0.05、P<0.01),大鼠肾脏病理形态变化明显。8周、12周T2DM肾病大鼠 BKCaɑ亚单位在肾动脉mRNA表达变化不明显;β1亚单位在肾动脉中表达下降(P<0.05、P<0.01)。结论 T2DM肾病大鼠肾动脉上BKCa通道β1亚单位mRNA表达降低,并与肾脏病变程度一致。
Abstract
OBJECTIVE To reveal the mRNA expression change of BKCa channel in renal artery in different course in T2DM nephropathy rats.METHODS T2DM nephropathy models were induced by one side nephrotomy, high-fat diet in rats and small doses of streptozotocin through intraperitoneal injection; Real-time PCR were performed to verify mRNA expression. Then body weight, 24 h urinary albumin, blood urea nitrogen(BUN),serum creatinine(Scr), kidney weight are measured. Renal tissue was observed under optical and electron microscope.RESULTS Blood glucose, blood insulin, serum creatinine, urea nitrogen, 24 h urinary albumin, kidney hypertrophy index of T2DM nephropathy rats in 8 weeks and 12 weeks, were significantly higher than that of control group (P<0.05, P<0.01);the rat kidney pathological morphological changes were obvious. Compared with the control group, ɑ subunit expression of BKCa in the model group at 8 weeks and 12 weeks were obviously unchanged;β1 subunit expression of BKCa were reduced(P<0.05,P<0.01).CONCLUSION The mRNA expression of BKCa are reduced in renal artery in different course, and this chang is consistent with the pathological changes degree of kidney in T2DM nephropathy rats.
关键词
2型糖尿病 /
肾脏病变 /
BKCa通道 /
mRNA表达变化
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Key words
type 2 diabetes mellitus /
renal lesion /
BKCachannel /
mRNA expression change
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中图分类号：
R965
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参考文献
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脚注
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基金
四川省教育厅科研基金重点项目资助(16ZA0184)
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